Protein Expression Analysis in A549 Cells

After drug intervention or no drug intervention, A549 or A549/DDP cells were lysed and subjected to western blot analysis as a general method [16 (link)]. After treatment, the blots were probed with antibodies against Beclin 1, LC3, p62, cleaved caspase-3, p53, Bax, Bcl-2, FOXO3a, p-FOXO3a Ser253, p-FOXO3a Ser315, and p-FOXO3a Thr32; then, we detected the protein bands with ECL solution (Vazyme, Nanjing, China) using a Tanon 5200 imaging system (Tanon Science & Technology, Shanghai China) [17 (link)].

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Zhou H., Pan P., Zhao Q., Liu W., Sun Y., Wang J., Liu C, & Wang C. (2022). Overcoming Basal Autophagy, Kangai Injection Enhances Cisplatin Cytotoxicity by Regulating FOXO3a-Dependent Autophagic Cell Death and Apoptosis in Human Lung Adenocarcinoma A549/DDP Cells. BioMed Research International, 2022, 6022981.

Publication 2022

ECL solution Tanon 5200 imaging system

A549 cells Antibodies Bcl 2 Beclin 1 Caspase 3 Drug Protein Western blot analysis

Corresponding Organization : Liaoning University of Traditional Chinese Medicine

Other organizations : Shenyang Medical College

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Variable analysis

independent variables

Drug intervention
No drug intervention

dependent variables

Beclin 1 expression
LC3 expression
P62 expression
Cleaved caspase-3 expression
P53 expression
Bax expression
Bcl-2 expression
FOXO3a expression
P-FOXO3a Ser253 expression
P-FOXO3a Ser315 expression
P-FOXO3a Thr32 expression

control variables

Cell lines: A549 and A549/DDP

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