All protocols followed the guidelines for the use of laboratory animals and were approved by the Institutional Animal Care and Usage Committee at Temple University.
This study used brain tissue of doxycycline induced GFAP promoter-driven HIV-1 Tat transgenic mice generously provided by Dr. Johnny He to the Comprehensive NeuroAIDS Center47 (link). Briefly, mice were divided into two groups, (+DOX) and (−DOX) with 4 animals per group. The doxycycline-induced Tat (iTat) animals were injected i.p. with doxycycline hyclate (DOX) (Sigma-Aldrich) at the dosage of 80 mg/kg/day for seven days. The control group was injected with saline (.09% NaCl) in the same manner as their Dox treated counterparts. After seven days, both groups of mice were sacrificed and brains were extracted/dissected into regions of interest (i.e. hippocampus, cerebellum, brain stem, and frontal cortex)55 (link). Total RNA was isolated for each brain region using Trizole reagent (Invitrogen, ThermoFisher, Carlsbad, CA), as previously described55 (link).
This study used brain tissue of doxycycline induced GFAP promoter-driven HIV-1 Tat transgenic mice generously provided by Dr. Johnny He to the Comprehensive NeuroAIDS Center47 (link). Briefly, mice were divided into two groups, (+DOX) and (−DOX) with 4 animals per group. The doxycycline-induced Tat (iTat) animals were injected i.p. with doxycycline hyclate (DOX) (Sigma-Aldrich) at the dosage of 80 mg/kg/day for seven days. The control group was injected with saline (.09% NaCl) in the same manner as their Dox treated counterparts. After seven days, both groups of mice were sacrificed and brains were extracted/dissected into regions of interest (i.e. hippocampus, cerebellum, brain stem, and frontal cortex)55 (link). Total RNA was isolated for each brain region using Trizole reagent (Invitrogen, ThermoFisher, Carlsbad, CA), as previously described55 (link).
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