The cells were seeded in a 24-well plate and added with EdU (ST067, Beyotime, Shanghai, China) at 10 µmol/L concentration. After incubation for 2 h, cells (6–10 fields) were randomly observed under a fluorescence microscope (FM-600, Shanghai Pudan Optical Instrument Co., Ltd.). The number of EdU-positive cells in each field was counted [53 (link)]. EdU labeling rate (%) was calculated as the number of positive cells/(number of positive cells + number of harmful cells) × 100%.
Other organizations :
Mianyang Central Hospital, University of Electronic Science and Technology of China, Affiliated Hospital of Southwest Medical University, Maternal and Child Health Hospital of Sichuan Province
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