The Saccharomyces cerevisiae strain, Kyokai No. 701, was obtained from the Brewing Society of Japan [10 (link)]. Yeast peptone dextrose (YPD) medium (1% yeast extract (Becton, Dickinson (BD) and Company, Sparks, MD, USA), 2% peptone (BD), and 2% glucose) was used for the cultivation of S. cerevisiae.
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