Anti-phospho-NF-κB p65 (Ser536) rabbit polyclonal antibody (pAb) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-NF-κB p65 rabbit pAb was obtained from Abcam (Cambridge, MA, USA). IκBα monoclonal antibody (mAb), His mAb, and Flag mAb were purchased from Cell Signaling Technology (Danvers, MA, USA). GAPDH mAb, tubulin mAb, VEGF mAb, smooth muscle actin (SMA) pAb, horseradish-peroxidase-labeled goat anti-mouse and goat anti-rabbit IgG were purchased from Santa Cruz Biotechnology (Santa Cruz). Western blotting was carried out as described previously (46 (link),47 (link)). IMD 0354, an IKKβ inhibitor, was obtained from Selleck Chemicals (Shanghai, China).
Protocol detail
Immunoblotting Assay for NF-κB Signaling
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Actin
Anti antibody
Anti igg
Antibody
Gapdh
Goat
Horseradish peroxidase
Ikkβ
Imd 0354
Iκbα
Monoclonal antibody
Mouse
Nf κb p65
Rabbit
Smooth muscle
Tubulin
Vegf
Corresponding Organization :
Other organizations : Nanjing Medical University, Quzhou College of Technology, Xuzhou Medical College, Affiliated Hospital of Xuzhou Medical College, UPMC Hillman Cancer Center, University of Southern California
Top 5 similar protocols
Variable analysis
independent variables
- IMD 0354, an IKKβ inhibitor
- NF-κB p65 (Ser536) phosphorylation
- NF-κB p65 expression
- IκBα expression
- VEGF expression
- Smooth muscle actin (SMA) expression
- GAPDH
- Tubulin
- Positive control: None specified
- Negative control: None specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!