HPV detection for high-risk HPV subtypes was performed on adenosquamous carcinomas in the tissue microarray that had sufficient tissue to score and had not been improperly fixed or stored (n=23). HPV in situ hybridization with a chromogen was performed using the Advanced Cell Diagnostics (Hayward, CA) RNAscope® system (catalogue no.312598). The RNAscope® Probe “HPV HR18” contains probes targeting E6 and E7 mRNA for the following high-risk subtypes: HPV16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82. The methodology and interpretation were discussed in detail in a previous paper (18 (link)). Positive and negative control cases were used for optimal results. A full range of cytoplasmic and nuclear signals were encountered. Cases were interpreted to be HPV-positive if any brown signal appeared (nuclear, cytoplasmic) in the presence of a negative control.
Protocol detail
High-Risk HPV Detection in Adenosquamous Carcinomas
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Adenosquamous carcinomas
Cell
Chromogen
Cytoplasmic
Diagnostics
Hpv16
In situ hybridization
Microarray
Mrna
Tissue
Corresponding Organization : Memorial Sloan Kettering Cancer Center
Other organizations : Universitatea de Medicină, Farmacie, Științe și Tehnologie „George Emil Palade” din Târgu Mureș, Vancouver General Hospital, Institutul Regional de Oncologie, Ospedale Sacro Cuore Don Calabria, Sheba Medical Center, Jikei University School of Medicine, Hospital General de México, Massachusetts General Hospital
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Variable analysis
independent variables
- HPV detection for high-risk HPV subtypes
- Presence of HPV (positive/negative)
- Adenosquamous carcinomas in the tissue microarray that had sufficient tissue to score and had not been improperly fixed or stored (n=23)
- Positive and negative control cases
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