All samples were stained as previously described by our laboratory(Hill et al. 2018a (link); Hill et al. 2018b (link)). Antibodies used were anti-Ki67 (1:500, Abcam, Cambridge, UK; Cat# ab15580 Lot# GR264777 RRID:AB_443209), anti-DCX (1:1000, Millipore, Burlington, MA, USA; Cat# AB2253 Lot# 2828588 RRID:AB_1586992), anti-BrdU (1:200, Abcam, Cat# ab6326 Lot# GR267766–1 RRID:AB_305426), anti-IBA-1 (1:500, Wako Chemicals, Richmond, VA, USA; Cat# 019–19741 Lot#WDK2121 RRID:AB_839504), and anti-CD68 (1:1000, Bio-Rad Laboratories, Hercules, CA, USA; Cat# MLA1957 Lot#1708 RRID:AB_322219). Secondary antibodies used were Alexa-488® goat anti-guinea pig (Thermo Fisher Scientific, Waltham, MA, USA; Cat# A-11073 Lot#1841755 RRID:AB_2534117), Alexa-594® donkey anti-rat (Thermo Fisher Scientific; Cat# A-21209 Lot#45081A RRID:AB_2535795), Alexa-488® donkey anti-rabbit (Thermo Fisher Scientific; Cat# A-21206 Lot#1608521 RRID:AB_2535792). All secondary antibodies were used at a dilution of 1:400. Imaging for in vivo quantification was performed using a CoolSNAP EZ CCD camera (Photometrics, Tucson, AZ, USA) coupled to a Nikon i80 Eclipse (Nikon Instruments Inc., Melville, NY, USA). Confocal images were taken on a Nikon A1R confocal microscope (Nikon).