Total RNA from adult mouse DG was isolated using the TRIzol reagent according to the manufacturer’s instructions (Invitrogen). mRNA purification was performed with poly(A)+ RNA selection twice using Dynabeads Oligo (dT)25 (Thermo Fisher; 61006). A total of 150 ng of mRNA was subjected to m6A-SMART-seq using anti-m6A rabbit polyclonal antibody (Synaptic Systems, 202003) as previously described9 (link). Briefly, 5 μg of anti-m6A polyclonal antibody was conjugated to Dynabeads Protein A (Thermo Fisher; 10001D) and used for each affinity pull-down. The m6A RNA was eluted twice with 6.7 mM N6-Methyladenosine (Sigma-Aldrich; M2780) in 1 × IP buffer (10 mM Tris-HCl (pH 7.5), 150 mM NaCl, and 0.1% (vol/vol) Igepal CA-630) and recovered by RNA Clean and Concentrator-5 (Zymo Research). Libraries were generated using the SMART-seq protocol as described35 (link). Three biological replicates for each condition were sequenced using Illumina NextSeq 550 from a single end for 75 bases.