Extraction and characterization of Orthosiphon stamineus leaf bioactive compounds

The preparation of UE-12 aqueous extract has been previously described (Kong et al., 2014 (link)). Briefly, Orthosiphon stamineus leaves were collected and taken through a series of stringent microbial tests as well as Atomic Absorption Spectrophotometry (AAS) to test for heavy metal presence. The leaves were then dried, ground into fine powder which was used in a water-based extraction. The microbial and trace metal element tests were repeated again on the extraction yield. The product was examined for the presence of functional groups/molecules by Fourier transform infrared spectroscopy (FTIR) and High-performance liquid chromatography (HPLC). Sinensetin (CAS no. 2306-27-6), eupatorin (CAS no. 855-96-9) and rosmarinic acid (CAS no. 20283-92-5) were purchased from ChromaDex®, Inc. (Irvine, CA). The extract and compounds were dissolved in dimethyl sulfoxide (DMSO) and filtered through a 0.2 µm membrane filter (Sartorius Stedim, Germany) followed by storage at −20°C in a desiccated storage container.

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Kong C., Tan M.W, & Nathan S. (2014). Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation. Biology Open, 3(7), 644-655.

Publication 2014

Sinensetin rosmarinic acid

Atomic absorption spectrophotometry Dimethyl sulfoxide Eupatorin Ftir Heavy metal High performance liquid chromatography Membrane Metal Orthosiphon stamineus Powder Rosmarinic acid Sinensetin Trace element

Corresponding Organization :

Other organizations : National University of Malaysia, Stanford University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Presence of functional groups/molecules
Identification and quantification of sinensetin, eupatorin, and rosmarinic acid

control variables

Microbial tests
Heavy metal presence tests (Atomic Absorption Spectrophotometry)
Procedure for drying, grinding, and extracting the Orthosiphon stamineus leaves

controls

Positive control: Sinensetin, eupatorin, and rosmarinic acid standards purchased from ChromaDex®, Inc.
Negative control: Not explicitly mentioned

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