MAGeT Brain was evaluated on a dataset of T2-weighted MRI data from 25 C57Bl/6 fixed mice brains. Images were gadolinium-enhanced to increase the contrast within the image. The specimen preparation for postmortem sample preparation has been described elsewhere [Dorr et al., 2008 (link); Lerch et al., 2011b (link)]. Briefly, a multichannel 7.0 T MRI scanner (Varian, Palo Alto, CA) with a 6-cm inner bore diameter insert gradient set was used to acquire anatomical images of brains within skulls. Prior to imaging, the samples were placed into 13-mm-diameter plastic tubes filled with a proton-free susceptibility-matching fluid (Fluorinert FC-77, 3M Corp., St. Paul, MN). Three custom-built, 14-mm-diameter solenoid coils with a length of 18.3 mm and over wound ends were used to image three brains in parallel. Parameters used in the scans were optimized for gray/white matter contrast: a T2-weighted, 3D fast spin-echo sequence, with TR/TE = 325/32 ms, four averages, field-of-view 14 × 14 × 25 mm3 and matrix size = 432 × 432 × 780 giving an image with 32 μm isotropic voxels. Total imaging time was 11.3 h. Geometric distortion due to position of the three coils inside the magnet was corrected using an MR phantom.