Organoid-Based Chemo-Sensitivity Screening

Organoids were utilized in chemo-sensitivity screens mimicking intravenous conditions during which these were treated with 5-fluorouracil (5-FU), oxaliplatin, irinotecan, FOLFOX, FOLFIRI, or regorafenib as described previously.^{11 (link)} Stock solutions of 10 mM were prepared for 5-FU (F6627; Sigma), irinotecan (I1406; Sigma), and regorafenib (S1178; Selleckchem) by dissolving in dime-thyl sulfoxide (DMSO), while oxaliplatin and folinic acid (47612; Sigma-Aldrich) were dissolved in deionized H₂O. Treatment solutions were prepared in cell culture media to the following concentrations: 10 μM 5FU, 1 μM oxaliplatin, 1 μM irinotecan, 1:10:1 μM folinic acid:5FU:oxaliplatin (FOLFOX), 1:10:1 μM folinic acid:5FU:oxaliplatin (FOLFIRI), and 1 μM regorafenib. After 7 days, spent media was removed from organoids, and treatments were added to the wells. Organoids were treated for 72 h, after which viability assays were performed.

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Forsythe S.D., Sasikumar S., Moaven O., Sivakumar H., Shen P., Levine E.A., Soker S., Skardal A, & Votanopoulos K.I. (2020). Personalized Identification of Optimal HIPEC Perfusion Protocol in Patient-Derived Tumor Organoid Platform. Annals of surgical oncology, 27(13), 4950-4960.

Publication 2020

irinotecan regorafenib F6627 oxaliplatin folinic acid

Assays Cell Cell culture Culture media Folinic acid Irinotecan Organoids Oxaliplatin Regorafenib Sensitivity Sulfoxide

Corresponding Organization :

Other organizations : Wake Forest University, Atrium Health Wake Forest Baptist, The Ohio State University, Forest Institute

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Variable analysis

independent variables

5-fluorouracil (5-FU)
Oxaliplatin
Irinotecan
FOLFOX
FOLFIRI
Regorafenib

dependent variables

Organoid viability

control variables

Cell culture media
Incubation time (72 h)

controls

Positive control: Not specified
Negative control: Not specified

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