Cells were transfected with 30nM miRVana microRNA antimiRs or mimics (Thermofisher Scientific, Waltham, MA, USA) with inhibitors and mimics (Supplementary Table S7 ) as described previously.56 (link) The NC Dicer-substrate siRNA (51-01-14-03, IDT) and PAX3-FOXO1 siRNA (detailed in Supplementary Table S4 ) were transfected with 3nM duplex as above.
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MicroRNA Interference in Cell Transfection
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Corresponding Organization :
Other organizations : St. Jude Children's Research Hospital
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Variable analysis
independent variables
- 30nM miRVana microRNA antimiRs or mimics (Thermofisher Scientific, Waltham, MA, USA) with inhibitors and mimics
- NC Dicer-substrate siRNA (51-01-14-03, IDT)
- PAX3-FOXO1 siRNA (detailed in Supplementary Table S4)
- Measured outcomes
- Kept constant to ensure valid results
- Positive control: Not specified
- Negative control: NC Dicer-substrate siRNA (51-01-14-03, IDT)
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