The human DLBCL cell lines OCI-Ly7 and OCI-Ly3 were purchased from the ATCC. The cell lines were routinely cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 2.0 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin. All cells were cultured in a humidified incubator at 37 °C with 5% CO2. The cells were transfected by using the transfection reagent Lip2000 in accordance with the manufacturer’s instructions. After transfection for 48 h, the cells were harvested for subsequent experiments.16 (link)
Protocol detail
DLBCL Cell Line Transfection Protocol
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Cell lines
Cells
Cultured medium
Fetal bovine serum
Glutamine
Human
Penicillin
Streptomycin
Transfection
Corresponding Organization :
Other organizations : Guangzhou Medical University, Guangzhou First People's Hospital, South China University of Technology
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Variable analysis
independent variables
- Transfection reagent Lip2000
- Subsequent experiments
- RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 2.0 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin
- Humidified incubator at 37 °C with 5% CO2
- Positive control: Not specified
- Negative control: Not specified
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