PI3K Activation via Transduction Peptide

Activation of PI3K was achieved by a transduction peptide (PTD4-PI3KAc) with the following sequence: YARAAARQARAGSDGGpYMDMS [29 (link),46 (link)–48 (link)]. This is a cell membrane permeable phosphopeptide composed of a transduction domain of the tat family [48 (link)] fused to a SH2 interacting domain that allows the activation of the class I PI3K independently from tyrosine kinase dimerization [29 (link),48 (link)–49 (link)]. Peptides were purchased either from GenScript (NJ, USA) or BioPeptide (FR). GSK3 inhibitors: SB-415286 and AR-A014418 [50 (link)–52 (link)] were from Tocris, UK (ref. 1617) and from Sigma, USA (ref. A3230), respectively.

Free full text: Click here

Cuesto G., Jordán-Álvarez S., Enriquez-Barreto L., Ferrús A., Morales M, & Acebes Á. (2015). GSK3β Inhibition Promotes Synaptogenesis in Drosophila and Mammalian Neurons. PLoS ONE, 10(3), e0118475.

Publication 2015

SB-415286 AR-A014418

Ar a014418 Cell membrane permeable Dimerization Gsk3 Inhibitors Peptides Phosphopeptide Pi3k Sb 415286 Sh2 domain Tyrosine kinase

Corresponding Organization :

Other organizations : Center for Biomedical Research of La Rioja, Instituto Cajal

Top 5 similar protocols

Variable analysis

independent variables

PTD4-PI3KAc peptide sequence: YARAAARQARAGSDGGpYMDMS
GSK3 inhibitors: SB-415286 and AR-A014418

dependent variables

Activation of PI3K

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!