The following strains were used in this work: PGRP-LE112 (Kaneko et al., 2004 (link)), PGRP-LCΔE12 (Gottar et al., 2002 (link)), DreddD55(Leulier et al., 2000 (link)), RelishE20 (Hedengren et al., 1999 (link)), pirkEY0073 (Lhocine et al., 2008 (link)), PGRP-LB∆(Paredes et al., 2011 (link)), Df(3R)ED5516 (BL#8968), Df(1)Excel6234 and TβHnM18 (kindly provided by Henrike Scholz). UAS-nlsGFP (BL#4775), UAS-IMD (Georgel et al., 2001 (link)), UAS-TTX (Sweeney et al., 1995 (link)), UAS-TRPA1 (Hamada et al., 2008 (link)), UAS-Kir2.1 (Hardie et al., 2001 (link)), QUAS-GFP (BL# 52264), UAS-TβH (kindly provided by Henrike Scholz) and UAS-FaddIR, (Khush et al., 2002 (link)) (Kindly provided by P. Meier), UAS-RelIR (BL#28943) and UAS-LEIR (BL#60038). Elav-Gal4 (BL# 8760), NP1-Gal4 (DGRC-Kyoto #112001), Tdc2-Gal4 and TβH-Gal4 (kindly provided by Henrike Scholz), ppk-Gal4 (kindly provided by Barry Dickson), ILp7-Gal4 (Kindly provided by Irene Miguel-Aliaga), prospero-Gal4 and Voila-Gal4 (kindly provided by Armel Gallet).
Flies were grown at 25°C on a yeast/cornmeal medium in 12 hr/12 hr light/dark cycle controlled incubators. For 1 L of food, 8.2g of agar (VWR, cat. #20768.361), 80g of cornmeal flour (Westhove, Farigel maize H1) and 80 g of yeast extract (VWR, cat.#24979.413) were cooked for 10 min in boiling water. 5.2 g of Methylparaben sodium salt (MERCK, cat.#106756) and 4 ml of 99% propionic acid (CARLOERBA, cat. #409553) were added when the food had cooled down. For antibiotic treatment (ATB), standard medium was supplemented with Ampicillin, Kanamycin, Tetracyclin and Erythromycin at 50 μg/ml final concentrations.
Flies were grown at 25°C on a yeast/cornmeal medium in 12 hr/12 hr light/dark cycle controlled incubators. For 1 L of food, 8.2g of agar (VWR, cat. #20768.361), 80g of cornmeal flour (Westhove, Farigel maize H1) and 80 g of yeast extract (VWR, cat.#24979.413) were cooked for 10 min in boiling water. 5.2 g of Methylparaben sodium salt (MERCK, cat.#106756) and 4 ml of 99% propionic acid (CARLOERBA, cat. #409553) were added when the food had cooled down. For antibiotic treatment (ATB), standard medium was supplemented with Ampicillin, Kanamycin, Tetracyclin and Erythromycin at 50 μg/ml final concentrations.
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