Zika and Sendai Virus Infections in Human Keratinocytes

Zika virus–Dakar (DAK; Zika virus/A.africanus-tc/SEN/1984/41525-DAK) (GenBank accession no. KU955591) was used for the in vitro experiments in human keratinocytes, and Zika virus–French Polynesia (PF; Zika virus strain H/PF/2013) (GenBank accession no. KJ776791.2) was used for in vivo infection studies. Strains were provided by S. Weaver at the University of Texas Medical Branch and H.L. (coauthor of this study), respectively. Sendai virus strain Cantell was obtained from Charles River Laboratory. Zika virus stocks were prepared as described (55 (link)). All viruses were titered by focus-forming assay in Vero cells. Vero cells (CCL-81) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) and were verified as mycoplasma free by the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich, St. Louis, MO, USA). In vitro Zika and Sendai virus infections were performed in serum-free media conditions for 1 hour, after which human keratinocyte medium with indicated treatment conditions was replenished.

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Kwock J.T., Handfield C., Suwanpradid J., Hoang P., McFadden M.J., Labagnara K.F., Floyd L., Shannon J., Uppala R., Sarkar M.K., Gudjonsson J.E., Corcoran D.L., Lazear H.M., Sempowski G., Horner S.M, & MacLeod A.S. (2020). IL-27 signaling activates skin cells to induce innate antiviral proteins and protects against Zika virus infection. Science Advances, 6(14), eaay3245.

Publication 2020

Sendai virus strain Cantell Vero cells (CCL-81) LookOut Mycoplasma PCR Detection Kit

Assay Conditions medium Human Infection Keratinocyte Mycoplasma River Sendai virus infections Serum free media Strain Vero cells Virus sen Viruses Zika

Corresponding Organization : Duke University

Other organizations : University of Michigan–Ann Arbor, University of North Carolina at Chapel Hill, Duke Institute for Health Innovation

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Variable analysis

independent variables

Zika virus strain (Zika virus–Dakar (DAK) and Zika virus–French Polynesia (PF))
Sendai virus strain Cantell

dependent variables

Infection outcomes in human keratinocytes

control variables

Serum-free media conditions for 1 hour during in vitro Zika and Sendai virus infections
Vero cells (CCL-81) from the American Type Culture Collection (ATCC), verified as mycoplasma free

controls

Positive control: Not specified.
Negative control: Not specified.

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