Embryos (3 dpf) were fixed in 4% paraformaldehyde in PBS (w/v) overnight at 4°C, and whole mount in-situ hybridization was performed as previously described [16 (link)]. The stained embryos were mounted in 3% methylcellulose in water (w/v). The mounted embryos were covered with a thin layer of PBST during stereo-microscopic (Leica MZ FLIII) imaging.
Free full text: Click here