Monocyte Isolation from Blood

Monocytes were isolated from a 30 ml Lithium-heparin-anti-coagulated blood sample in accordance to a previously described protocol [57 (link)]. In particular, Ficoll-based density gradient centrifugation to extract peripheral blood mononuclear cells was followed by magnetic cell sorting (autoMACS, Miltenyi Biotec, Bergisch Gladbach, Germany) using CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Purity of the isolated monocytes was ensured by flow cytometry (FACSVerse^™ flow cytometer, BD Bioscience, Heidelberg, Germany) using an anti-human-CD14-FITC antibody (BioLegend, San Diego, USA).

Free full text: Click here

Siegler B.H., Altvater M., Thon J.N., Neuhaus C., Arens C., Uhle F., Lichtenstern C., Weigand M.A, & Weiterer S. (2021). Postoperative abdominal sepsis induces selective and persistent changes in CTCF binding within the MHC-II region of human monocytes. PLoS ONE, 16(5), e0250818.

Publication 2021

autoMACS CD14 MicroBeads FACSVerse™ flow cytometer anti-human-CD14-FITC antibody

Anti antibody Blood Density gradient centrifugation Ficoll Fitc Flow cytometry Heparin Human Lithium Microbeads Monocytes Peripheral blood mononuclear cells

Corresponding Organization : Lukaskrankenhaus

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Isolation of monocytes from a 30 ml Lithium-heparin-anti-coagulated blood sample
Ficoll-based density gradient centrifugation to extract peripheral blood mononuclear cells
Magnetic cell sorting (autoMACS) using CD14 MicroBeads

dependent variables

Purity of the isolated monocytes

control variables

Previously described protocol for isolating monocytes [57]
Flow cytometry (FACSVerse^™ flow cytometer) using an anti-human-CD14-FITC antibody to ensure purity of isolated monocytes

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!