One day before transfection, cells were seeded onto collagen-coated glass cover slip. At 48 h post-transfection, the cells were fixed with 4% paraformaldehyde and stained as described previously [41 (link)]. Alexa Fluor-conjugated secondary antibodies (Life Technologies) were used to detect signals. Microscopic imaging was performed with an FV1000-D confocal laser scanning microscope (Olympus, Tokyo, Japan).
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