The HCV infectivity titers were measured by indirect immunostaining as described previously21 (link),46 (link). For the indirect immunofluorescence analysis, the cells were fixed with cold methanol and incubated with an anti-core antibody (clone C7–50; Abcam, Cambridge, UK) and subsequently with fluorescent labeled secondary antibody. The infectivity titer was calculated the focus-forming units per mL and expressed as relative to JFH1/5AC-wt.
Nitta S., Asahina Y., Kato T., Tsuchiya J., Inoue-Shinomiya E., Sato A., Tsunoda T., Miyoshi M., Kawai-Kitahata F., Murakawa M., Itsui Y., Nakagawa M., Azuma S., Kakinuma S., Hikita H., Takehara T, & Watanabe M. (2019). Impact of novel NS5A resistance-associated substitutions of hepatitis C virus detected in treatment-experienced patients. Scientific Reports, 9, 5722.
Cells were incubated with an anti-core antibody (clone C7–50; Abcam, Cambridge, UK)
Cells were subsequently incubated with fluorescent labeled secondary antibody
controls
Positive control: JFH1/5AC-wt
Negative control: Not specified
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