GST, 4G10 and phospho-(S79)-ACC, antibodies were from Millipore (Billerica, MA, USA), 6xHis, phospho-(T172)-AMPK, total-AMPK and total-ACC antibodies were from Cell Signaling (Danvers, MA,USA), LKB1 antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA,USA) and Millipore (Billerica, MA,USA), GAPDH and tubulin alpha antibodies were from Abcam (Cambridge, MA, USA). Flag antibody was from Sigma-Aldrich (St. Louis, MO, USA), and the V5-epitope antibody was from MBL international (Woburn, MA, USA). Phospho LKB1 tyrosine 261 and 365 specific antibodies (pY261- and pY365-LKB1) were developed in our laboratory [17] (link). GST-Fyn fusion protein was purchased from Millipore (Billerica, MA, USA). TAT-LKB1 modified peptides (11R-WT and 11R-Sca) were synthesized by BIOMATIK (Wilmington, DE, USA). pcDNA3-Fyn-WT, pcDNA3-Flag-LKB1-WT and pcDNA3-Flag-LKB1-Y261/365F were constructed and described before [17] (link). pGEX was a generous gift from Dr. Bridget Shafit-Zagardo (Albert Einstein College of Medicine, NY,USA). C2C12 muscle cells were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA).
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