Immunofluorescence Staining of Drosophila

Mouse anti-SCAR (DSHB P1C1-SCAR RRID:AB_2618386) was used at a dilution of 1:100. Rat anti HA (Roche catalog number: 11867423001 RRID:AB_390918) was used at a dilution of 1:1000. Guinea pig anti-Non-stop (Mohan et al., 2014a (link)) was used at a dilution of 1:150. Mouse anti-Chaoptin (deposited to the DSHB by Benzer, S and Colley, N.) (DSHB 24B10 RRID:AB_528161) was used at a dilution of 1:250. Goat anti-Rat IGG −568 (Invitrogen Catalog number: A-11077 RRID:AB_141874) was used at a dilution of 1:1000. Goat anti-Mouse IGG-488 (Invitrogen Catalog number: A-11001 RRID:AB_2534069) was used at a dilution of 1:1000. Goat anti-Guinea pig IGG- 488 (Invitrogen Catalog number: A11073 RRID:AB_142018) was used at a dilution of 1:1000. Phalloidin-488 (Invitrogen Catalog number: A12379 RRID:AB_2759222) was used at a dilution of 1:20. Phalloidin-568 (Invitrogen Catalog number: A12380 RRID:AB_2810839) was used at a dilution of 1:20.