CSF Biomarkers Measurement in Dementia

A lumbar puncture with measurement of the CSF core biomarkers Aβ₄₂, T-tau and P-tau using the ELISA technique with the Innotest kit (Innogenetics, Ghent, Belgium) was performed for all patients. The analysis was done at the laboratory at Akershus University Hospital, Norway. The laboratory is a part of the Alzheimer´s Association QC program for CSF biomarkers [22 ]. The recommended cut-off value for a normal test was until June 2017 >550 pg/mL for Aβ₄₂. As of October 2018, when these data were collected, the recommended cut-off values for a normal test from the laboratory were Aβ₄₂ >700 pg/mL [23 (link)], P-tau <80 pg/mL and T-tau <300 pg/mL for patients below 50 years, < 450 pg/mL for patients aged 50 to 69 years, and <500 pg/mL for patients older than 70 years. These cut-off values were used to support the clinical diagnoses that were made (see section below).