Protein Extraction and Western Blot Analysis

Protein extraction and Western blot analysis of whole mouse cerebellar and cortical tissue was performed as previously described [29 (link)]. The following primary antibodies were used: mouse anti-Gapdh (Sigma G8795, 1:10,000, St. Louis, MO, USA), mouse anti-Vinculin (Millipore v9264 1:10,000, Burlington, MA, USA), rabbit anti-Gfap (Sigma G4546, 1:500), rabbit anti-Iba1 (Wako, 019-19741, 1:400), rabbit anti-Prkcb (Thermo Fisher Scientific 12919-1-AP, 1:1000, Waltham, MA, USA), rabbit anti-Camkk2 (Thermo Fisher Scientific 11549-1-AP, 1:1000).

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Luttik K., Olmos V., Owens A., Khan A., Yun J., Driessen T, & Lim J. (2022). Identifying Disease Signatures in the Spinocerebellar Ataxia Type 1 Mouse Cortex. Cells, 11(17), 2632.

Publication 2022

mouse anti-Gapdh mouse anti-Vinculin rabbit anti-Gfap rabbit anti-Iba1

Antibodies Cerebellar Cortical Gapdh Gfap Mouse Prkcb Protein Rabbit Tissue Vinculin Western blot analysis

Corresponding Organization : Yale Cancer Center

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Variable analysis

independent variables

Tissue type (cerebellar and cortical)

dependent variables

Protein expression levels of Gapdh, Vinculin, Gfap, Iba1, Prkcb, and Camkk2

control variables

Experimental procedures (protein extraction and Western blot analysis) as previously described [29]

controls

Positive controls: Not specified
Negative controls: Not specified

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