Glucose-Induced Confocal Imaging of INS-1 Cells

INS-1 832/13 cells were incubated (for 24 h) with either basal (LG; 2.5 mM) or high (HG; 20 mM) glucose on chamber slides. Samples were prepared for confocal imaging according to previously published protocols from our laboratory [28 (link)]. Images were obtained by a 63×oil objective utilizing a Zeiss Axio Examiner Z1 upright microscope. Image analysis was completed using Volocity 7.0 software [27 (link)]. All confocal microscopy studies were conducted at the Microscopy, Imaging, and Cytometry Resources Core at Wayne State University School of Medicine.

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Gleason N, & Kowluru A. (2024). Hyperglycemic Stress Induces Expression, Degradation, and Nuclear Association of Rho GDP Dissociation Inhibitor 2 (RhoGDIβ) in Pancreatic β-Cells. Cells, 13(3), 272.

Publication 2024

Axio Examiner Z1

Corresponding Organization : Wayne State University

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Variable analysis

independent variables

Glucose concentration: basal (2.5 mM) or high (20 mM)

dependent variables

Unspecified (not explicitly mentioned)

control variables

Incubation time: 24 hours
Cell line: INS-1 832/13 cells
Microscopy technique: Confocal imaging using a 63×oil objective on a Zeiss Axio Examiner Z1 upright microscope
Image analysis software: Volocity 7.0

controls

No positive or negative controls were explicitly mentioned in the provided information.

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