Visualizing M. tuberculosis Lung Lesions

A subset of live M. tuberculosis-infected animals from the relapse cohort were imaged within a sealed bio-containment bed (Minerve) modified in-house to be compliant with biosafety level 3 (BSL3) containment, as described previously [7 (link), 19 (link)]. A standard small animal anesthesia machine was used to deliver a mixture of isoflurane and oxygen to anesthetize during imaging. Computed tomography (CT) scans were then immediately performed using the NanoSPECT/CT (Mediso) in vivo animal imager. The images were analyzed using VivoQuant 2.5 (Invicro). The whole lung field was segmented and the frequency of voxels with a given density (measured as Hounsfield Units, HU) was determined. A cavity was defined as a macroscopic region of air (density <−900 HU) within diseased lung parenchyma.

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Ordonez A.A., Pokkali S., Kim S., Carr B., Klunk M.H., Tong L., Saini V., Chang Y.S., McKevitt M., Smith V., Gossage D.L, & Jain S.K. (2018). Adjunct antibody administration with standard treatment reduces relapse rates in a murine tuberculosis model of necrotic granulomas. PLoS ONE, 13(5), e0197474.

Publication 2018

NanoSPECT/CT VivoQuant 2.5

Anesthesia Animal Cavity Computed tomography Isoflurane Lung M tuberculosis Oxygen Relapse Scans

Corresponding Organization : Gilead Sciences (United States)

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Variable analysis

independent variables

Subset of live Mycobacterium tuberculosis-infected animals from the relapse cohort

dependent variables

Frequency of voxels with a given density (measured as Hounsfield Units, HU) in the whole lung field
Presence of a cavity defined as a macroscopic region of air (density <−900 HU) within diseased lung parenchyma

control variables

Anesthesia: Isoflurane and oxygen mixture delivered using a standard small animal anesthesia machine
Imaging: Computed tomography (CT) scans performed using the NanoSPECT/CT (Mediso) in vivo animal imager
Image analysis: Images analyzed using VivoQuant 2.5 (Invicro)

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