Characterization of C. perfringens Mutant

Fluorescein isothiocyanate (FITC)- and phycoerythrin (PE)-conjugated specific antibodies against mouse CD11b (clone M1/70) or Ly-6G (clone 1A8) and purified rat anti-mouse CD16/CD32 (Fc Block) were purchased from BD Biosciences (San Jose, CA, USA). Recombinant human G-CSF, filgrastim, was from Mochida Pharmaceutical Co., Ltd. (Tokyo, Japan). All other chemicals were of the highest grade available from commercial sources. C. perfringens wild-type (WT) Strain 13 was used in this study. The preparation of a plc gene-knockout mutant of C. perfringens (PLC-KO) was as described in our previous report [16 (link)].

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Takehara M., Sonobe Y., Bandou H., Kobayashi K, & Nagahama M. (2019). Granulocyte Colony-Stimulating Factor Does Not Influence Clostridium Perfringens α-Toxin-Induced Myonecrosis in Mice. Toxins, 11(9), 509.

Publication 2019

purified rat anti-mouse CD16/CD32 (Fc Block)

Antibodies C perfringens Cd11b Clone Filgrastim Fluorescein G csf Gene knockout Human Isothiocyanate Mouse Pharmaceutical Phycoerythrin Strain

Corresponding Organization : Tokushima Bunri University

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Variable analysis

independent variables

Knockout of the plc gene in C. perfringens (PLC-KO)
Treatment with recombinant human G-CSF (filgrastim)

dependent variables

Not explicitly mentioned

control variables

C. perfringens wild-type (WT) Strain 13
Purified rat anti-mouse CD16/CD32 (Fc Block) for flow cytometry analysis

controls

Positive control: C. perfringens wild-type (WT) Strain 13
Negative control: C. perfringens plc gene-knockout mutant (PLC-KO)

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