Homogenisation was performed according to the methodology described in the previous studies [14 (link), 16 (link)]. The samples of the tumour and NT were weighed and homogenised using a PRO200 homogeniser (PRO Scientific Inc., Oxford, CT, USA) at 10,000 rpm in 9 volumes of PBS (EURx, Gdansk, Poland). After homogenisation, sonification with an ultrasonic cell disrupter was performed (UP100H, Hielscher Ultrasonics GmbH, Teltow, BB, Germany).
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Tissue Homogenization for Tumor Analysis
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Corresponding Organization :
Other organizations : Medical University of Silesia
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Variable analysis
independent variables
- Homogenisation methodology
- Tumor samples
- Normal tissue (NT) samples
- Homogenisation speed (10,000 rpm)
- Homogenisation buffer (9 volumes of PBS)
- Sonification with an ultrasonic cell disrupter (UP100H)
- No positive or negative controls were explicitly mentioned.
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