Several commercially available primary antibodies were utilized: RAD51D (Novus), XRCC2 (Santa Cruz), BRCA1 (Millipore) and BRCA2 (Calbiochem). Anti-RAD51C, anti-XRCC3, anti-γH2AX, anti-HA, and anti-β actin antibodies were as described elsewhere.[24 (link)] Secondary antibodies for immunofluorescence microscopy and the detection of immunoblots using chemiluminescence (Amersham) were as described previously.[23 (link)]
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Park J.Y., Virts E.L., Jankowska A., Wiek C., Othman M., Chakraborty S.C., Vance G.H., Alkuraya F.S., Hanenberg H, & Andreassen P.R. (2016). COMPLEMENTATION OF HYPERSENSITIVITY TO DNA INTERSTRAND CROSSLINKING AGENTS DEMONSTRATES THAT XRCC2 IS A FANCONI ANEMIA GENE. Journal of medical genetics, 53(10), 672-680.
Other organizations :
Cincinnati Children's Hospital Medical Center, Riley Hospital for Children, Indiana University, Heinrich Heine University Düsseldorf, King Saud University, University School, King Faisal Specialist Hospital & Research Centre, Alfaisal University, University of Duisburg-Essen, University of Cincinnati Medical Center
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