In accordance with our previous research [38 (link)], the collected tissue samples (heart, liver, spleen, kidney, and brain) were fixed in 4% paraformaldehyde solution, dehydrated in 50–100% ethanol and distilled water, then paraffin embedded, cut into 5 μm slices, dewaxed twice in xylene, twice in 100–75% anhydrous ethanol, and finally hydrated. All specimens were stained with H&E. The pathological status of the collected tissue samples was analyzed, sections were observed, and images were collected using a standing optical microscope (Eclipse E100, Nikon, Tokyo, Japan).
As in our previous study [39 (link)], after dewaxing and hydration, the brain sections were added to a citric acid antigen retrieval buffer (pH 6.0) (G1202, Servicebio, Beijing, China) for antigen repair. Tissues were evenly covered with 3% bovine serum albumin and incubated at room temperature for 30 min. The tissues were then left overnight along with antibody Aβ1–42 (bs-0107R, Bioss, Beijing, China). The phosphate buffer solution (PBS) was washed thrice before incubation with horseradish peroxidase-labeled goat anti-rabbit antibody (E-AB-1003, Elabscience, Wuhan, China) at 25 °C for 1 h. The sections were then restrained with diaminobenzidine tetrachloride solution and hematoxylin and observed under a microscope (Eclipse E100).
As in our previous study [39 (link)], after dewaxing and hydration, the brain sections were added to a citric acid antigen retrieval buffer (pH 6.0) (G1202, Servicebio, Beijing, China) for antigen repair. Tissues were evenly covered with 3% bovine serum albumin and incubated at room temperature for 30 min. The tissues were then left overnight along with antibody Aβ1–42 (bs-0107R, Bioss, Beijing, China). The phosphate buffer solution (PBS) was washed thrice before incubation with horseradish peroxidase-labeled goat anti-rabbit antibody (E-AB-1003, Elabscience, Wuhan, China) at 25 °C for 1 h. The sections were then restrained with diaminobenzidine tetrachloride solution and hematoxylin and observed under a microscope (Eclipse E100).
Free full text: Click here
