Multimodal Neuroinflammation Quantification

According to (Abbas et al. 2021 (link)), 6 non-overlapping fields in CA1 and cerebral cortex were chosen randomly in each sample and examined for detecting relative area percentage of immunohistochemical expression of GFAP; mean reactive microglial cells counts for CD163, CD86 and Iba-1 in immunohistochemically stained sections. Besides, the mean count of intact neurons in CA1 and cortex in toluidine blue stained tissue sections was performed. Also, the number of Ferric ion deposits was detected by Prussian blue stain in CA1 and cerebral cortex.Lastly, the optical density (OD) of CD163 expressed in neuron cells of CA1 and cortex was measured.
The Leica Application module linked to the imaging system (Leica Microsystems GmbH, Germany) was used to examine and obtain data.