Genomic DNA Extraction and Sequencing Library Preparation
The genomic DNA was extracted from 200 μl blood samples with the QIAamp DNA Blood Mini kit (Qiagen) and from the skin samples with a standard phenol–chloroform method. The quality and integrity of DNA was controlled by OD260/280 ratio and agarose gel electrophoresis. For sequencing library preparation, the genomic DNA was sheared to fragments of 300–500 bp, which were then end repaired, ‘A’-tailed, and ligated to Illumina sequencing adapters. The ligated products with sizes of 370–470 bp were selected on 2% agarose gels and then amplified by PCR. The libraries were sequenced on Illumina HiSeq platform with standard paired-end mode.
Ming L., Yuan L., Yi L., Ding G., Hasi S., Chen G., Jambl T., Hedayat-Evright N., Batmunkh M., Badmaevna G.K., Gan-Erdene T., Ts B., Zhang W., Zulipikaer A., H.o.s.b.l.i.g., E.r.d.e.m.t., Natyrov A., Mamay P., N.a.r.e.n.b.a.t.u., Meng G., Narangerel C., Khongorzul O., He J., Hai L., Lin W., S.i.r.e.n.d.a.l.a.i., S.a.r.e.n.t.u.y.a., A.i.y.i.s.i., Li Y., Wang Z, & J.i.r.i.m.u.t.u. (2020). Whole-genome sequencing of 128 camels across Asia reveals origin and migration of domestic Bactrian camels. Communications Biology, 3, 1.
Corresponding Organization : Shanghai Industrial Technology Institute
Other organizations :
Inner Mongolia Agricultural University, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai Institutes for Biological Sciences, Mongolian University of Science and Technology, University of Mohaghegh Ardabili, Mongolian Academy of Sciences, Institute of Chemistry and Chemical Technology, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Xinjiang Academy of Animal Science, Kalmyk State University, Kazakh National Agrarian Research University, Inner Mongolia Medical University, New Mongol Institute of Technology
Sequencing platform (Illumina HiSeq with standard paired-end mode)
control variables
Blood sample volume (200 μl)
Gel electrophoresis conditions (2% agarose gels)
controls
Positive control: None mentioned
Negative control: None mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
