Quantification of Phytochemical Compounds

The PMFs content was determined using ultrahigh-performance liquid chromatography (UPLC, Waters ACQUITYI-Class (Milford, MA, USA) equipped with a photodiode array detector, a quaternary solvent delivery system, and a column temperature controller). Chromatographic separations were performed on a 2.1 × 100 mm, 1.7 µm ACQUITY UPLC HSS C18 column (Waters, MA, USA). The mobile phase consisted of water/formic acid (99.99%: 0.01%, v/v) (A) and methanol (B) at the rate of 0.4 mL/min, and the gradient profile was as a previous study reported [19 (link)]. All the prepared samples were tested in triplicate.