Multiplexed Bioluminescence and Fluorescence Imaging

Multiplexed bioluminescence/fluorescence imaging was performed as previously described (Brancaccio et al, 2013 (link)), using the LV200 system (Olympus Microscopy, UK). Briefly, SCN explants were sealed into 35 mm dishes with glass bottoms (Mattek, Slovakia) and transferred to the heated stage of an LV200 microscope system equipped with an EM‐CCD camera (Hamamatsu, Japan). For combined bioluminescence and fluorescence, images were taken at a time resolution of 30 min. Fluorescence exposure was 100 ms, and bioluminescence signal was acquired over 29.5 min.

Free full text: Click here

Morris E.L., Patton A.P., Chesham J.E., Crisp A., Adamson A, & Hastings M.H. (2021). Single‐cell transcriptomics of suprachiasmatic nuclei reveal a Prokineticin‐driven circadian network. The EMBO Journal, 40(20), e108614.

Publication 2021

LV200 system glass bottoms EM‐CCD camera

Dishes Fluorescence Microscope

Corresponding Organization :

Other organizations : MRC Laboratory of Molecular Biology, University of Manchester

Top 5 similar protocols

Variable analysis

independent variables

Multiplexed bioluminescence/fluorescence imaging

dependent variables

Bioluminescence signal
Fluorescence signal

control variables

SCN explants sealed into 35 mm dishes with glass bottoms
Heated stage of an LV200 microscope system
EM-CCD camera (Hamamatsu, Japan)
Fluorescence exposure time of 100 ms
Bioluminescence signal acquired over 29.5 min

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!