Total lung cells were harvested at day 7 p.i. after mock or RSV infection as previously described (17 (link), 19 (link)). Isolated cells were incubated with anti-FcγRII/FcγRIII mAb (24G2; BD Biosciences). For cell-surface marker staining, an aliquot of cells was stained with the following anti-mouse antibodies: anti-CD11c, anti-F4/80, anti-CD11b, and anti-Gr-1 (all from BD-Pharmingen). Samples were stained at 4°C in PBS with 1 % FBS and analyzed with a FACS Canto flow cytometer equipped with BD FACSDiva software (both from Becton Dickinson Immunocytometry Systems). Analysis was performed using WinMDI2.8 (Scripps).
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