Tissues were harvested from three to five mice for each genotype (WT, HPV16tg/+; HPV16tg/+;Krt17−/−) at each time point (1-, 3- and 6 months) post implantation. For each mouse, both ears and the cervix were removed, quickly embedded in Tissue-Tec OCT compound (Sakura Finetek, Torrance, CA, USA) and stored at − 20 °C prior to sectioning. In total, 5–7 μM-thick tissue sections were subjected to histological stains as described.22 (link) Tissues used for RNA in situ hybridization were immediately fixed in freshly prepared 4% paraformaldehyde prior to embedding and processed for Aire in situ hybridization as described.22 (link)
Protocol detail
Analyzing Tissue Samples from Transgenic Mice
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Johns Hopkins University
Top 5 similar protocols
Variable analysis
independent variables
- Genotype (WT, HPV16^(tg/+), HPV16^(tg/+);Krt17^(-/-))
- Time point (1-, 3- and 6 months) post implantation
- Histological staining of tissue sections
- RNA in situ hybridization for Aire expression
- Tissue sources (ears and cervix)
- Tissue embedding (Tissue-Tec OCT compound)
- Tissue sectioning (5–7 μM-thick)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!