Ovarian Cancer Cells Selenium Treatment

SKOV-3 and OVCAR-3 cells were cultured in growth medium to 80%confluency on sterile plastic petri dishes (Corning, UK) at 37 °C and 5% CO2. After 48 h incubation culture medium was removed and 2 mL of fresh medium containing aqueous Se (Na₂SeO₃) or SeNPs. SKOV-3 cells were treated with IC20; selenite 3 μg/mL, BSA-SeNP 6 μg/mL or chitosan-SeNP 13 μg/mL. OVCAR-3 cells were treated with selenite 40 μg/mL, BSA-SeNP 20 μg/mL or chitosan-SeNP 18 μg/mL. Following addition of selenium treatment cells were incubated for a further 48 h prior to analysis. A minimum of three biological repeats were conducted for each cell type and treatment.