Immunohistochemical Staining of Cryosections

Cryosections 7um in thickness were cut and fixed in cold acetone.
Sections were stained with anit-ICAM1 (3E2; BD Biosciences); anti-VCAM1 (429; BD
Biosciences); anti-MADCAM1(MECA367; Bio X Cell); anti-IgD (11-26c.2a;
Biolegend); anti-Vitronectin (347317; R&D); rat IgG2a isotype control
(2A3; Bio X Cell) or anti-CD35 (8C12; BD Biosciences) using described protocols
(36 (link)).

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Wang X., Rodda L., Bannard O, & Cyster J.G. (2014). Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4601-4609.

Publication 2014

anti-VCAM1 (429; anti-MADCAM1(MECA367; anti-IgD (11-26c.2a; rat IgG2a isotype control(2A3; anti-CD35 (8C12;

Acetone Anti igd Cd35 Cell Cold Cryosections Icam1 Igg2a Isotype Vitronectin

Corresponding Organization : University of California, San Francisco

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Variable analysis

independent variables

Staining with anti-ICAM1 (3E2; BD Biosciences)
Staining with anti-VCAM1 (429; BD Biosciences)
Staining with anti-MADCAM1(MECA367; Bio X Cell)
Staining with anti-IgD (11-26c.2a; Biolegend)
Staining with anti-Vitronectin (347317; R&D)
Staining with rat IgG2a isotype control (2A3; Bio X Cell)
Staining with anti-CD35 (8C12; BD Biosciences)

dependent variables

Not explicitly mentioned

control variables

Cryosections 7um in thickness
Sections were fixed in cold acetone

positive controls

Not explicitly mentioned

negative controls

Rat IgG2a isotype control (2A3; Bio X Cell)

Annotations

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