Retinal Protein Expression Analysis

Freshly isolated retinal tissues were homogenized and lysed in RIPA buffer (P0013; Beyotime, Beijing, China). The protein concentration was quantified by a Bicinchoninic Acid kit (Pierce, Rockford, IL, USA). Western blotting was done as described in our previous study (20 (link)) with the following antibodies: anti-PPARα (ab215270; Abcam; 1:1,000), anti-GFAP (ab33922; Abcam; 1:1,000), anti-COX2 (ab62331; Abcam; 1:1,000), and anti-β-actin (GB12001; Servicebio; 1:3,000). The expression levels of PPARα, GFAP and COX2 were quantified by Image-Pro Plus 6.0 (Media Cybernetics, Rockville, MD, USA) and normalized by the densitometry of β-actin.