Quantifying Nutrient Ions in Meltwater

Assays for putative nutrients such as NH₄⁺, NO₂^–, NO₃^–, SO₄^2–, soluble reactive phosphorus (SRP), and dissolved organic carbon (DOC) from each filtered meltwater sample were performed by ion chromatography in an 861 Advance Compact IC system (Metrohm AG, Herisau, Switzerland). Ions in ice samples were identified and quantified with internal and external standards prepared from Certified Standard Solutions (TraceCERT^®) (Merck). Chromatograms were analyzed with the Metrohm IC Net 2.3 SR4 software (Garcia-Lopez et al., 2022 (link)). Detection limits for these constituents ranged from 0.1 to 2.0 μM. Concentrations of ions were analyzed by inductively coupled plasma-mass spectrometry (ICP-MS) on a Perkin Elmer ELAN9000 ICP-MS quadrupole spectrometer (Martinez-Alonso et al., 2019 (link)). Values > 0.999 ppb were considered for the statistical test.

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Ruiz-Blas F., Muñoz-Hisado V., Garcia-Lopez E., Moreno A., Bartolomé M., Leunda M., Martinez-Alonso E., Alcázar A, & Cid C. (2023). The hidden microbial ecosystem in the perennial ice from a Pyrenean ice cave. Frontiers in Microbiology, 14, 1110091.

Publication 2023

861 Advance Compact IC system TraceCERT ELAN9000 ICP-MS quadrupole spectrometer

Assays Chromatography Dissolved organic carbon Ions Mass spectrometry Nutrients Organic Phosphorus Plasma

Corresponding Organization : Centro de Astrobiología

Other organizations : Helmholtz Centre Potsdam - GFZ German Research Centre for Geosciences, Instituto Pirenaico de Ecología, University of Cologne, Swiss Federal Institute for Forest, Snow and Landscape Research, Oeschger Centre for Climate Change Research, University of Bern, University of the Basque Country, Instituto Ramón y Cajal de Investigación Sanitaria

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Concentrations of NH4+, NO2–, NO3–, SO42–, soluble reactive phosphorus (SRP), and dissolved organic carbon (DOC)

control variables

None explicitly mentioned

controls

Internal and external standards prepared from Certified Standard Solutions (TraceCERT®) were used to identify and quantify ions in ice samples.
A detection limit range of 0.1 to 2.0 μM was specified for the measured constituents.
Values > 0.999 ppb were considered for the statistical test.

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