Transgenic Mouse Models for Stem Cell Research

Nes-Gfp (ref. 20 (link)), FVB-Tg(Col1a1-cre)1Kry/Mmcd (ref. 29 (link)), B6.Cg(SJL)-TgN(Nes-cre)1Kln (ref. 31 (link)), Nes-Cre^ERT2 (ref. 32 (link)), RCE:loxP (ref. 33 (link)), C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J^{39 (link)}, B6.129S4-Gt(ROSA)26Sortm1Sor/J^{30 (link)} transgenic mice (Jackson Laboratories) and C57BL/6-CD45.1 mice (Frederick Cancer Research Center) have been used in these studies. Detailed procedures for cell isolation and culture, in vitro differentiation, LT-CIC and heterotopic bone ossicle assays, histological analyses of ossicles, in vivo treatments and cell depletion experiments, homing assay, RNA isolation, quantitative real-time RT–PCR, gene chip and analyses of microarray experiments, gene ontology and protein–protein interactions are available in the Methods.
Full Methods and any associated references are available in the online version of the paper at www.nature.com/nature.

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Méndez-Ferrer S., Michurina T.V., Ferraro F., Mazloom A.R., MacArthur B.D., Lira S.A., Scadden D.T., Ma’ayan A., Enikolopov G.N, & Frenette P.S. (2010). Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature, 466(7308), 829-834.

Publication 2010

Assay Bone C57bl mice Cancer Cell Cell isolation procedures Gene chip Hbegf Heterotopic Isolation Microarray analyses Mmcd Protein Quantitative real time pcr Rosa Transgenic mice

Corresponding Organization : Albert Einstein College of Medicine

Other organizations : Cold Spring Harbor Laboratory, Massachusetts General Hospital, Harvard University

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Protocol cited in 37 other protocols

Variable analysis

independent variables

Nes-Gfp
FVB-Tg(Col1a1-cre)1Kry/Mmcd
B6.Cg(SJL)-TgN(Nes-cre)1Kln
Nes-Cre^ERT2
RCE:loxP
C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
B6.129S4-Gt(ROSA)26Sortm1Sor/J

dependent variables

Detailed procedures for cell isolation and culture, in vitro differentiation, LT-CIC and heterotopic bone ossicle assays, histological analyses of ossicles, in vivo treatments and cell depletion experiments, homing assay, RNA isolation, quantitative real-time RT–PCR, gene chip and analyses of microarray experiments, gene ontology and protein–protein interactions

control variables

C57BL/6-CD45.1 mice

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

Annotations

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