Statistical Analysis of Neuroprotective Compounds

All the raw data from behavioral testing, histological, and immunohistochemical parts of the study were summarized in Excel (Office 2010, Microsoft). The statistical comparison of the four tested groups (EGCG, PP242, combination of the two, and vehicle-treated tested group) was accomplished with several statistical tests in the GraphPad Prism for Windows software (version 5.03, GraphPad Software, Boston, MA, USA). For evaluation of the normality of the raw data, Kolmogorov–Smirnov test was used. For behavioral testing, the two-way repeated measurement ANOVA was applied, except for the Ladder walking test, where the one-way ANOVA was used. Immunohistochemical GFAP analysis was analyzed with the two-way ANOVA mixed-effects model (REML). The Bonferroni correction test was used as a post hoc pair-to-pair test. The tests were selected according to the distribution of the data for each investigation.
All graphs in the results part display as arithmetical means with standard error of the mean. The statistical significance in the graphs as well as in the text is signposted by a scheme; * p < 0.05, ** p < 0.01, *** p < 0.001.