Immunoblotting was performed on cell lysates or precipitates from cell supernatants as described before (2 (link), 47 (link)) with the following the antibodies; caspase-1 p20 (clone casper1; Adipogen), IL-1β (AF-401-NA; R&D Systems), gasdermin D (EPR19828; Abcam), mouse caspase-11 (17D9; Cell Signaling), Stx2 A subunit (11E10; Thermo Scientific), human gasdermin D (EPR19829; Abcam), IL-18 (Biovision), and β-actin (Sigma-Aldrich).
Protocol detail
Immunoblotting for Inflammasome Proteins
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Variable analysis
independent variables
- Immunoblotting was performed on cell lysates or precipitates from cell supernatants
- Levels or presence of the following proteins: caspase-1 p20, IL-1β, gasdermin D, mouse caspase-11, Stx2 A subunit, human gasdermin D, IL-18, and β-actin
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned in the provided information.
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