FOXO3 Genotyping and Longevity

DNA was isolated from the buffy coat of peripheral blood using the PureGene system (Gentra Systems, Minneapolis, MN) and quantified using PureGreen staining (Molecular Probes, Eugene, OR). We selected the FOXO3 longevity-associated allele rs2802292, which has been shown to be strongly correlated with coronary artery disease mortality and longevity in Japanese and other ethnicities (4 (link),5 (link),8 (link),18 (link)). The genotype for SNP rs2802292 (G—longevity associated; TT—not associated with longevity) was assessed using the standard TaqMan PCR method and commercial TaqMan Assays. G-allele carriers represent those participants who carry at least one copy of the G allele (TG or GG genotype).

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Klinpudtan N., Allsopp R.C., Kabayama M., Godai K., Gondo Y., Masui Y., Akagi Y., Srithumsuk W., Sugimoto K., Akasaka H., Takami Y., Takeya Y., Yamamoto K., Ikebe K., Yasumoto S., Ogawa M., Ishizaki T., Arai Y., Rakugi H., Chen R., Willcox B.J., Willcox D.C, & Kamide K. (2021). The Association Between Longevity-Associated FOXO3 Allele and Heart Disease in Septuagenarians and Octogenarians: The SONIC Study. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 77(8), 1542-1548.

Publication 2021

PureGene system

Allele Assays Coronary artery disease Ethnicities Genotype Japanese Molecular probes Peripheral blood buffy coat

Corresponding Organization : Osaka University

Other organizations : University of Hawaiʻi at Mānoa, Biogen (United States), Osaka University of Human Sciences, Tokyo Metropolitan Geriatric Hospital, Keio University, Kuakini Medical Center, Okinawa International University

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Variable analysis

independent variables

FOXO3 longevity-associated allele rs2802292

dependent variables

Genotype for SNP rs2802292 (G—longevity associated; TT—not associated with longevity)

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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