Genetically Modified Mice for Immunological Studies

All male and female mice used for experiments were between 6 and 12 weeks old; age-matched littermates were used. The RORγt^−/− (Rorc^tm1Litt, stock no. 007571) mouse strain was described previously (18 (link)). The RORγt^K256R/K256R point-mutated mice were designed and generated by Biocytogen LLC. Rag1^−/− (Rag1^tm1Mom, stock no. 002216), Tg^Tcr2D2 (Tcra2D2 and Tcrb2D2, stock no. 006912), IL-17A–GFP (Il17a^tm1Bcgen, stock no. 018472), CRISPR-Cas9– enhanced GFP (EGFP) [Gt(ROSA)26Sor^{em1.1(CAG-cas9}*^,-EGFP)Rsky, stock no. 028555], and C57BL/6J (stock no. 000664) mice were purchased from the Jackson Laboratory. For some assays, the mice were crossed to generate RORγt^−/−/Tg^Tcr2D2, RORγt^K256R/K256R/Tg^Tcr2D2, and RORγt^K256R/K256R/IL-17A-IRES-GFP-KI mice. All mice were bred at the C57BL/6J background and maintained in a pathogen-free animal facility at City of Hope. All animal experiments were conducted per the protocols approved by the Institutional Animal Care and Use Committee at City of Hope. Statistical tests were not used to predetermine sample sizes. The sample sizes were chosen on the basis of previous studies of our own and by others in the field (8 (link)). The sample sizes are indicated in the figure legends or figures. Allocation of mice to experimental groups was random.