Enzymatic Synthesis of TG-DHA for Neuroprotection

Triglyceride of docosahexaenoic acid (TG-DHA) obtained by enzymatic synthesis was kindly supplied by Brudy Technology (Barcelona, Spain). This oil contains more than 70% TG-DHA and more than 90% of ω-3 of total fatty acids (Mancera et al., 2017 (link)). The stock TG-DHA solution in 100% ethanol was diluted with the indicated aqueous buffer before use. All other compounds were obtained from external sources: 6-hydroxydopamine (6-OHDA) (Sigma-Aldrich, St. Louis, MO, United States) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium (MTT) (Sigma-Aldrich). The primary antibodies used were rabbit anti-TH polyclonal antibody (Millipore, Temecula CA, United States) and rabbit anti-α-actinin-1 polyclonal antibody (Santa Cruz biotechnology, Dallas TX, United States). The secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Pierce Biotechnology, Rockford, IL, United Staes) and Alexa Fluor 488-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc., West Grove, PA, United States).

Free full text: Click here

Gómez-Soler M., Cordobilla B., Morató X., Fernández-Dueñas V., Domingo J.C, & Ciruela F. (2018). Triglyceride Form of Docosahexaenoic Acid Mediates Neuroprotection in Experimental Parkinsonism. Frontiers in Neuroscience, 12, 604.

Publication 2018

6-hydroxydopamine (6-OHDA) rabbit anti-TH polyclonal antibody Alexa Fluor 488-conjugated donkey anti-rabbit IgG

6 ohda Actinin Alexa fluor 488 Anti antibody Anti igg Antibodies Bromide Buffer Docosahexaenoic acid Donkey Enzymatic Ethanol Goat Horseradish peroxidase Rabbit Synthesis Tetrazolium Triglyceride ω 3 fatty acids

Corresponding Organization : Institut d'Investigació Biomédica de Bellvitge

Top 5 similar protocols

Variable analysis

independent variables

Triglyceride of docosahexaenoic acid (TG-DHA) obtained by enzymatic synthesis

dependent variables

Cell viability (measured by MTT assay)

control variables

Aqueous buffer used to dilute the stock TG-DHA solution in 100% ethanol
6-hydroxydopamine (6-OHDA)
Primary antibodies: rabbit anti-TH polyclonal antibody and rabbit anti-α-actinin-1 polyclonal antibody
Secondary antibodies: horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG and Alexa Fluor 488-conjugated donkey anti-rabbit IgG

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!