Synthesis and Characterization of Inositol Phosphates

5-PP-InsP₄, 5-PCF₂Am-InsP₅ and 5-PCP-InsP₅ were synthesized as previously described (26 (link), 27 (link), 41 (link)). 3-PCP-InsP₅ was synthesized as previously described for 1,5-[PCP]2-InsP₄ but starting from 1d-6-O-benzyl-1,2:4,5-di-O-isopropylidene myo-inositol (42 (link)). The inositol pyrophosphates 1-InsP₇, 3-InsP₇ and 5-InsP₇ were synthesized using similar methods to those previously described (43 (link), 44 (link)). All synthetic compounds were purified by ion-exchange chromatography, fully characterized by ¹H, ³¹P and ¹³C NMR spectroscopy and where feasible quantified by total phosphate analysis. Commercially available InsP₆ (MilliporeSigma) was utilized.

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Chin A.C., Gao Z., Riley A.M., Furkert D., Wittwer C., Dutta A., Rojas T., Semenza E.R., Felder R.A., Pluznick J.L., Jessen H.J., Fiedler D., Potter B.V., Snyder S.H, & Fu C. (2020). The inositol pyrophosphate 5-InsP7 drives sodium-potassium pump degradation by relieving an autoinhibitory domain of PI3K p85α. Science Advances, 6(44), eabb8542.

Publication 2020

InsP6

13c nmr Inositol Insp5 Insp7 Ion exchange chromatography Isopropylidene Nmr spectroscopy Phosphate Pyrophosphates Spectroscopy

Corresponding Organization : Tianjin Medical University

Other organizations : University of Oxford, Leibniz-Forschungsinstitut für Molekulare Pharmakologie, University of Freiburg, University of Virginia

Top 5 similar protocols

Variable analysis

independent variables

5-PP-InsP4
5-PCF2Am-InsP5
5-PCP-InsP5
3-PCP-InsP5
1-InsP7
3-InsP7
5-InsP7
InsP6

dependent variables

Purification by ion-exchange chromatography
Characterization by 1H, 31P and 13C NMR spectroscopy
Quantification by total phosphate analysis

control variables

Synthesis methods used in previous studies (references 26, 27, 41, 42, 43, 44)

controls

Positive control: Commercially available InsP6 (MilliporeSigma)

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