RNA from adenovirus-infected cells was isolated by using PureLink RNA Mini Kit (Invitrogen) following manufacturer’s recommendations. Two to four micrograms of RNA were to reverse transcribed in a 20 μl reaction using oligo(dT)20 (link) and SuperScript III First-Strand Synthesis System (Invitrogen). Relative Quantitative Real Time PCR was performed in a total reaction volume of 10 μl, using 2 μl cDNA, 1 μl gene specific primer mix (QuantiTect primer Assays), 5 μl SYBR GreenER qPCR SuperMix (Invitrogen), and 2 μl water. The quantification of gene expression was performed using 7900HT Fast Real-Time PCR System (Applied Biosystems) in triplicate. The thermal profile of the reaction was: 50°C for 2 min, 95°C for 10 min and 35 cycles of 95°C for 15 s followed by 60°C for 1 min. Amplification of the sequence of interest was normalized with a reference endogenous gene Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The value was expressed as a fold change relative to RNA from cells infected with control adenovirus (Ad. Null). For data analysis the 7900HT Fast Real-Time PCR System Software was used (Applied Biosystems).