Retrograde Labeling of Propriospinal Neurons

The long propriospinal neurons (LPSNs) and short propriospinal neurons (SPSNs) were labeled with the retrograde tracer Fluoro-Ruby (MW, 10,000; 10% Fluoro-Ruby in PBS; Invitrogen) 2 weeks before the animals were perfused. Tracer injection procedures were modified slightly from a previous study^{34 (link)}. Briefly, the animals were anesthetized and placed in a stereotaxic frame. To label the LPSNs and SPSNs, 0.6 µL/site or 0.4 µL/site Fluoro-Ruby was bilaterally infused into the spinal cords at vertebral level T12 or C7 (lateral, ±0.8 mm, depth of 0.9 mm), respectively.

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Nakanishi T., Fujita Y, & Yamashita T. (2019). Neuropilin-1-mediated pruning of corticospinal tract fibers is required for motor recovery after spinal cord injury. Cell Death & Disease, 10(2), 67.

Publication 2019

Fluoro-Ruby

Animals Fluoro ruby Frame Neurons Spinal cords Vertebral

Corresponding Organization :

Other organizations : Osaka University

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Variable analysis

independent variables

Tracer injection site (T12 or C7)

dependent variables

Labeling of long propriospinal neurons (LPSNs) and short propriospinal neurons (SPSNs) with the retrograde tracer Fluoro-Ruby

control variables

Volume of Fluoro-Ruby injected (0.6 µL/site or 0.4 µL/site)
Injection depth (0.9 mm)
Injection laterality (±0.8 mm)
Time between tracer injection and animal perfusion (2 weeks)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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