THP-1 phagocytosis assay was performed as described before (47 (link)). In brief, biotinylated antigens were coupled to fluorescent neutravidin beads (Thermo Fisher) and incubated with 50 μl of 1:200 diluted plasma. THP-1 monocytes were added to the beads, incubated for 16 h at 37°C, fixed with 4% paraformaldehyde, and analyzed by flow cytometry on a LSR Fortessa (BD).
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